GFP-tagged balancer chromosomes for Drosophila melanogaster
نویسندگان
چکیده
We constructed green fluorescent protein (GFP)-expressing balancer chromosomes for each of the three major chromosomes of Drosophila melanogaster. Expression of GFP in these chromosomes is driven indirectly by a Kruppel (Kr) promoter, via the yeast GAL4-UAS regulatory system. GFP fluorescence can be seen in embryos as early as the germ band extension stage, and can also be seen in larvae, pupae, and adults. We show the patterns of GFP expression of these balancers and demonstrate the use of the balancers to identify homozygous progeny.
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ورودعنوان ژورنال:
- Mechanisms of Development
دوره 88 شماره
صفحات -
تاریخ انتشار 1999